Apoptosis and Autophagy
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
ご登録代理店情報 カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更は こちら。 |
| #2775S | 100 μL | 46,000 | |
|
LC3B抗体製品一覧
2775 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
| | |
-
2775:
-
Flow
Immunofluorescence*
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、免疫蛍光細胞染色 (IF-IC) (1:400)、フローサイトメトリー (1:100) |
| 種交差性 | |
| ヒト、マウス、ラット、(サル、ウシ、ブタ) |
| 特異性・感度 | |
| 内在性レベルのLC3B タンパク質を検出します。他のLC3 タンパク質のアイソフォームとも交差反応する可能性があります。LC3B タンパク質のタイプⅡと強く反応します。 |
| 検出タンパク質の分子量 | |
| 14 kDa、16 kDa |
| 使用抗原 | |
| LC3B タンパク質のN末端近傍領域 (合成ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with rat LC3B, and from HT-1080 and A20 cells, untreated or chloroquine-treated (50 μM, overnight), using LC3B Antibody.
Flow Cytometry
Flow cytometric analysis of Hela cells using LC3B Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or chloroquine-treated (right), using LC3B Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084(fluorescent DNA dye).
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form LC3-II have been used as indicators of autophagy (11).
-
Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.
-
Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-1518.
-
Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-2688.
-
Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-11497.
-
Lang, T. et al. (1998) EMBO J. 17, 3597-3607.
-
Kabeya, Y. et al. (2000) EMBO J. 19, 5720-5728.
-
He, H. et al. (2003) J. Biol. Chem. 278, 29278-29287.
-
Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-47710.
-
Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-442.
-
Ichimura, Y. et al. (2000) Nature 408, 488-492.
-
Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-2812.
- Li, C. et al. (2009) J Mol Cell Biol Applications: Western Blotting
- Chen, Y.F. et al. (2009) Genes Dev 23, 1183-94. Applications: Western Blotting
- Balakumaran, B.S. et al. (2009) Cancer Res 69, 7803-10. Applications: Western Blotting
- Mabuchi, S. et al. (2009) Clin Cancer Res 15, 5404-13. Applications: Western Blotting
- Deuretzbacher, A. et al. (2009) J Immunol 183, 5847-60. Applications: IF-IC (In Cells) Western Blotting
- Roca, H. et al. (2009) J Biol Chem 284, 34342-54. Applications: Western Blotting
- Alexander, A. et al. (2010) Proc Natl Acad Sci U S A 107, 4153-8. Applications: Western Blotting
- Wen, H. et al. (2011) Nat Immunol 12, 408-15. Applications: IF-IC (In Cells)
PhosphoSitePlus®：
