Cell Cycle / Checkpoint Control
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #2853S | 100 μL | 57,000 | |
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ATR抗体製品一覧
推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2853:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| 内在性レベルのSer428 がリン酸化されたATR タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのATR タンパク質のSer428 周辺領域 (合成ペプチド) |
Western Blotting
Western blot analysis of Raw264.7, SV-T2 and HT-29 cells that were untreated or UV-treated (50 mJ, 30 min), using Phospho-ATR (Ser428) Antibody. Lambda phosphatase NEB #P0753 (10,000 Units/ml, 1hr) was used to demonstrate the phospho-specificity of the antibody.
Western Blotting
Western blot analysis of untreated, UV-treated (50 mJ, 30 min) and nocodazole-treated (50 ng/ml, 24hr) Raw264.7 cells, using Phospho-ATR (Ser428) Antibody (upper) and a total ATR antibody (lower).
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI-3 Kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. While there have been no published reports of phosphorylation sites on ATR, Cell Signaling Technology has produced an antibody directed against phospho-ATR (Ser428) that demonstrates
in vivo
and UV-induced phosphorylation of this protein. This reagent could prove to be a valuable tool for monitoring ATR activation. Proline-directed phosphorylation sites like this one are often targeted by CDKs and MAPKs and can often dramatically affect protein conformation (4,5).
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Kastan, M.B. and Lim, D.S. (2000)
Nat. Rev. Mol. Cell Biol.
1, 179-186.
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Abraham, R.T. (2004)
DNA Repair (Amst)
3, 883-887.
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Shechter, D. et al. (2004)
DNA Repair (Amst)
3, 901-908.
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Pinna, L.A. and Ruzzene, M. (1996)
Biochim. Biophys. Acta
1314, 191-225.
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Zhou, X.Z. et al. (1999)
Cell Mol. Life Sci.
56, 788-806.
