Translational Control
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年7月30日15時15分 現在 |
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|---|---|---|---|---|
| #2855S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #2855L | 300 μL | 137,000 |
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推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫組織染色 (パラフィン) (1:1,600)、免疫蛍光細胞染色 (IF-IC) (1:200)、フローサイトメトリー (1:100) | |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、キイロショウジョウバエ | |
| 特異性・感度 | |
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| 内在性レベルのThr37/46 の両方およびそのどちらか一方がリン酸化された4E-BP1 タンパク質を検出します。また、同様の部位がリン酸化された4E-BP2 および4E-BP3 タンパク質と交差する可能性があります。 | |
| 検出タンパク質の分子量 | |
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| 15-20 kDa | |
| 使用抗原 | |
|---|---|
| マウスの4E-BP1 タンパク質のThr37/46 周辺領域 (合成リン酸化ペプチド) | |
| 抗体の由来 | |
|---|---|
| ウサギ | |
| 貯法 | |
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| -20℃ | |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody #9452 (upper) and Phospho-4E-BP1 (Thr37/46) Antibody #2855 (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lymphoma using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb on SignalSlide (TM) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells untreated (left) or LY294002-treated (right)).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb in the presence of control peptide (left) or Phospho-4E-BP1 (Thr37/46) Blocking Peptide #1052 (right).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002, Wortmannin and U0126-treated (blue), using Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells treated with LY294002 (left) or 20% serum (right) and labeled with Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
| バックグラウンド |
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Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
| 使用文献 |
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- Engelman, J.A. et al. (2008) Nat. Med. 14, 1351-1356. Applications: IHC-P (paraffin)
- Cao, R. et al. (2008) Mol. Cell. Neurosci. 38, 312-324. Applications: IHC-P (paraffin)
本製品は試験研究用です。
