Chromatin Regulation / Acetylation
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #2902S | 100 μL | 46,000 | |
|
Asf1b抗体製品一覧
推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2902:
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Immunofluorescence
Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング（1:1,000）、免疫沈降（1:100）、免疫蛍光細胞染色（IF-IC）（1:100） |
| 特異性・感度 | |
| 内在性レベルのASF1B タンパク質を検出します。ASF1A タンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのASF1B タンパク質のC末端領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from HeLa and HCT116 cells using ASF1B (C70E2) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using ASF1B (C70E2) Rabbit mAb (green). Actin filaments have been labeled with DY554 phalloidin (red).
ASF1 was first identified in
S. cerevisiae
based on its ability to de-repress transcriptional silencing when overexpressed (1). While only one gene exists in yeast and
Drosophila
, mammalian cells contain the two highly homologous ASF1A and ASF1B genes (2). ASF1A and ASF1B function as histone chaperones, delivering histone H3/H4 dimers to CAF-1 or HIRA histone deposition complexes to facilitate replication-coupled and replication-independent nucleosome assembly on DNA (2-5). Both ASF1A and ASF1B bind to CAF-1, but only ASF1A binds to HIRA (5). In addition to playing a role in DNA replication and gene silencing, ASF1 functions in DNA damage repair, genome stability and cellular senescence. Deletion of ASF1 in yeast and
Drosophila
confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint (6-8). Depletion of both ASF1A and ASF1B in mammalian cells results in the accumulation of cells in S phase, increased phosphorylation of H2A.X, centrosome amplification and apoptosis (9,10). ASF1A is required for the formation of senescence-associated heterochromatin foci (SAHF), with overexpression of ASF1A inducing senescence in primary cells (4). Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to replicative stress (11,12). The function of ASF1 phosphorylation is not yet understood.
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