Translational Control
p70 S6 Kinase Substrates Antibody Sampler Kit |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #2903S | 1 Kit | 91,000 | ログインすると国内在庫状況がご確認いただけます。
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2903 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 2708:
- IHC / Paraffin Western Blotting
- 3591:
- Western Blotting
- 3691:
- Immunoprecipitation Western Blotting
- 4858:
- Flow IHC / Frozen IHC / Paraffin Immunofluorescence Western Blotting
- 5364:
- Flow IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
- 9234:
- Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 | 40 µl | W IHC-P IHC-F IF-IC F | H M R Mk Sc (C) | 32 | Rabbit IgG |
| Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb #5364 | 40 µl | W IP IHC-P IF-IC F | H M R Mk | 32 | Rabbit IgG |
| Phospho-eIF4B (Ser422) Antibody #3591 | 40 µl | W | H M R Mk | 80 | Rabbit |
| Phospho-eEF2k (Ser366) Antibody #3691 | 40 µl | W IP | H R Mk | 105 | Rabbit |
| Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 | 40 µl | W | H M R Mk | 70, 85 | Rabbit IgG |
| p70 S6 Kinase (49D7) Rabbit mAb #2708 | 40 µl | W IHC-P | H M R Mk | 70, 85 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from PC12, NIH/3T3, and SK-N-MC cells, using p70 S6 Kinase (49D7) Rabbit mAb #2708.
Western Blotting
Western blot analysis of extracts from HeLa cells using Phospho-eIF4B (Ser422) Antibody #3591 (upper) or eIF4B Antibody #3592 (lower). 48 hours following siRNA transfection, cells were treated with Rapamycin (50 nM) and U0126 (10 µM) as indicated.
Western Blotting
Western blot analysis of extracts from SW-13 cells (starved for 18 hours) treated with calf intestinal alkaline phosphatase (CIP) or 20% fetal bovine serum for 30 minutes, and extracts from HeLa cells (starved for 18 hours) treated with 200 nM TPA for 30 minutes, using Phospho-eEF2k (Ser366) Antibody #3691 (upper) or eEF2k Antibody #3692 (lower).
Western Blotting
Western blot analysis of extracts from PC12 and NIH/3T3 cells, treated with λ phosphatase, FBS or PDGF as indicated, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).
Western Blotting
Western blot analysis of extracts from MCF7 and NIH/3T3 cells, treated with 100 nM insulin (10 min) or 20% FBS (30 min) as indicated, using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb #5364 (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).
Western Blotting
Western blot analysis of extracts from serum starved or serum treated (20%) 293, NIH/3T3, and PC12 cells, using Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 (upper), or p70 S6 Kinase (49D7) rabbit mAb #2708 (lower).
| バックグラウンド |
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p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, Ser244) located wtihin a small, carboxy-terminal region of the S6 protein (3,4). p70 S6 kinase has been shown to phosphorylate eIF4B at the rapamycin-sensitive site Ser422 in vivo, and a Ser422Ala mutant of eIF4B shows diminished activity in an in vitro translation assay (5). Phosphorylation of eEF2K by p70 S6 kinase and p90RSK leads to inactivation of eEF2K (6), facilitating the dephosphorylation of eEF2 and thus promoting translation.
- Pullen, N. and Thomas, G. (1997) FEBS Lett 410, 78-82.
- Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
- Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
- Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.
- Raught, B. et al. (2004) EMBO J 23, 1761-9.
- Wang, X. et al. (2001) EMBO J 20, 4370-9.
| 使用例 | |
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本製品は試験研究用です。