Transcription Factors
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #2911S | 100 μL | 57,000 | |
|
Catenin-delta抗体製品一覧
推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2911:
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Immunofluorescence
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫蛍光細胞染色（IF-IC）（1:100） |
| 特異性・感度 | |
| 内在性レベルのTyr228 がリン酸化されたCatenin δ-1 タンパク質を検出します。他の過剰発現させたチロシンがリン酸化されたタンパク質と交差反応する可能性があります。 |
| 検出タンパク質の分子量 | |
| 95 kDa、100 kDa |
| 使用抗原 | |
| ヒト/マウスのCatenin δ-1 タンパク質のTyr228 周辺領域（合成リン酸化ペプチド） |
Western Blotting
Western blot analysis of extracts from A431 cells, serum-starved overnight and then either left untreated or treated with EGF for 15 minutes, using Phospho-Catenin δ-1 (Tyr228) Antibody (upper) and Catenin δ-1 Antibody #4989 as a loading control (lower).
IF-IC
Confocal immunofluorescent analysis of A431 cells, serum-starved (left) or EGF-treated (right), using Phospho-Catenin δ-1 (Tyr228) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten, linked 42 amino-acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4).
Catenin δ-1 is phosphorylated at multiple tyrosine sites along its sequence both
in vivo
and
in vitro
(5). High levels of catenin δ-1 phosphorylated at Tyr228 are commonly seen in several carcinoma cell lines. EGFR signaling induces catenin δ-1 phosphorylation at Tyr228, with the phosphorylated protein becoming localized at adherens junctions although phosphorylation is not essential in junction formation (6).
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Reynolds, A.B. and Roczniak-Ferguson, A. (2004)
Oncogene
23, 7947-7956.
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Davis, M. A. et al. (2003)
J. Cell Biol.
163, 525-534.
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Thoreson, M.A. and Reynolds, A.B. (2002)
Differentiation
70, 583-589.
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Anastasiadis, P.Z. and Reynolds, A.B. (2000)
J. Cell Sci.
113, 1319-1334.
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Mariner, D.J. et al. (2001)
J. Biol. Chem.
276, 28006-28013.
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Mariner, D.J. et al. (2004)
J. Cell Sci.
117, 1339-1350.
