Cell Cycle / Checkpoint Control
|
|
| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年7月30日15時15分 現在 |
|
|---|---|---|---|---|
| #2914S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
|
|
推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
|---|
| 用途（希釈倍率） | |
|---|---|
| ウェスタンブロッティング（1:2,000）、免疫蛍光細胞染色（IF-IC）（1:50）、フローサイトメトリー（1:50） | |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット | |
| 特異性・感度 | |
|---|---|
| 内在性レベルのThr288 がリン酸化されたAurora A タンパク質、Thr232 がリン酸化されたAurora B タンパク質、Thr198 がリン酸化されたAurora C タンパク質を検出します。 | |
| 検出タンパク質の分子量 | |
|---|---|
| 35 kDa、40 kDa、48 kDa | |
| 使用抗原 | |
|---|---|
| ヒトのAurora B タンパク質のThr232 周辺由来の配列（合成ペプチド） | |
| 抗体の由来 | |
|---|---|
| ウサギ | |
| 貯法 | |
|---|---|
| -20℃ | |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from HeLa, L929 and C6 cells, treated with 4 mM hydroxyurea for 20 hours to induce G1/S phase or treated with 100 nM paclitaxel or 100 ng/ml nocodazole for 20 hours to induce G2/M phase, using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D11A13) XP™ Rabbit mAb (upper) or Aurora B/AIM1 Antibody #3094 (lower).
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP™ Rabbit mAb compared to propidium iodide (DNA content). The boxed population indicates phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)-positive cells.
IF-IC
Confocal immunofluorescent analysis of HT-1080 cells using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP™ Rabbit mAb (green), β-Tubulin (9F3) Rabbit mAb (Alexa Fluor ® 555 Conjugate) #2116 (red), and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (blue).
| バックグラウンド |
|---|
Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, Aurora B and Aurora C (1,2). Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Their functional influences span from G2 to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning and ingression (3). Aurora A is detected at the centrosomes, along mitotic spindle microtubules and in the cytoplasm of mitotically proliferating cells. Aurora A protein levels are low during G1 and S phases and peak during the G2/M phase of the cell cycle. Phosphorylation of Aurora A at Thr288 in its catalytic domain increases kinase activity. Aurora A is involved in centrosome separation, maturation and spindle assembly and stability. Expression of Aurora B protein also peaks during the G2/M phase of the cell cycle, while kinase activity peaks at the transition from metaphase to the end of mitosis. Aurora B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of both Aurora A and Aurora B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation (4,5), while overexpression of both kinases is seen in a variety of human cancers (2,4). Aurora C localizes to the centrosome from anaphase to cytokinesis and both mRNA and protein levels peak during G2/M phase. Although typical Aurora C expression is limited to the testis, overexpression of Aurora C is detected in various cancer cell lines (6).
- Warner, S.L. et al. (2003) Mol. Cancer Ther. 2, 589-595.
- Katayama , H. et al. (2003) Cancer Metastasis Rev. 22, 451-464.
- Andrews, P.D. et al. (2003) Curr. Opin. Cell Biol. 15, 672-683.
- Pascreau, G. et al. (2003) Prog. Cell Cycle Res. 5, 369-374.
- Crosio, C. et al. (2002) Mol. Cell. Biol. 22, 874-885.
- Kimura, M. et al. (1999) J. Biol. Chem. 274, 7334-7340.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。
