Antibody Sampler Kits
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月7日15時25分 現在 |
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| #2951S | 1 Kit | 91,000 | ログインすると国内在庫状況がご確認いただけます。
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2951 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 4176:
- Immunofluorescence Immunoprecipitation Western Blotting
- 9561:
- Western Blotting
- 9565:
- Western Blotting
- 9566:
- Immunoprecipitation Western Blotting
- 9582:
- IHC / Frozen IHC / Paraffin Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| β-Catenin (6B3) Rabbit mAb #9582 | 40 µl | W IP IHC-P IHC-F | H M R Mk (X) (B) (Dg) (Pg) (Hr) | 92 | Rabbit IgG |
| Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561 | 40 µl | W | H M R Mk (C) (X) (Z) (B) (Dg) (Pg) | 92 | Rabbit |
| Phospho-β-Catenin (Thr41/Ser45) Antibody #9565 | 40 µl | W | H M Mk (Pg) | 92 | Rabbit |
| Phospho-β-Catenin (Ser552) Antibody #9566 | 40 µl | W IP | H M (R) (C) (X) (Z) | 92 | Rabbit |
| Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176 | 40 µl | W IP IF-F IF-IC | H (M) (R) (C) (X) (Z) | 92 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from 293 cells, pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561.
Western Blotting
Western blot analysis of extracts from 293 cells pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Thr41/Ser45) Antibody #9565.
Western Blotting
Western blot analysis of total cell lysates from SK-N-MC cells, treated with Forskolin (FSK) for 30 minutes, or the lysate was treated with λ phosphatase for 1 hour, using Phospho-β-Catenin (Ser552) Antibody #9566.
Western Blotting
Western blot analysis of extracts from MKN-45 and SK-N-MC cells, untreated or treated with λ phosphatase for 1 hour or forskolin (FSK) for 30 minutes, using Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176(upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).
Western Blotting
Western blot analysis of total cell lysates from 293, Rat2 and COS cells using β-Catenin (6B3) Rabbit mAb #9582.
| バックグラウンド |
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β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
Both Akt and PKA were shown to phosphorylate β-catenin at Ser552 and Ser675. Phosphorylation at Ser552 and Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9-12).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
- Taurin, S. et al. (2006) J. Biol. Chem. 281, 9971-9976.
- Fang, D. et al. (2007) J. Biol. Chem. 282, 11221-11229.
- He, X.C. et al. (2007) Nat. Genet. 39, 189-198.
- Hino, S. et al. (2005) Mol. Cell Biol. 25, 9063-9072.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。