Translational Control
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #2972S | 100 μL | 46,000 | |
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mTOR抗体製品一覧
2972 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2972:
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Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング(1:1,000)、免疫沈降(1:100) |
| 特異性・感度 | |
| 内在性レベルのmTOR タンパク質を検出します。ウェスタンブロッティングでは、いくつかの他のタンパク質とわずかに交差します。 |
| 使用抗原 | |
| ヒトのmTOR タンパク質のSer2481 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from 293 cells (starved for 16 hours), untreated or EGF-treated (100 ng/ml), using Phospho-mTOR (Ser2448) antibody #2971 (upper) or mTOR Antibody (lower).
Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with either control siRNA (-) or mTOR siRNA (+). mTOR was detected using mTOR Antibody #2972, and eIF4B was detected using eIF4B Antibody #3592. The mTOR Antibody confirms silencing of mTOR expression, and the eIF4B Antibody is used to control for loading and siRNA specificity.
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
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Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
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Brown, E.J. et al. (1994) Nature 369, 756-8.
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Sabatini, D.M. et al. (1994) Cell 78, 35-43.
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Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
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Dennis, P.B. et al. (2001) Science 294, 1102-5.
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Fang, Y. et al. (2001) Science 294, 1942-5.
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Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
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Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
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Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.
- Suzuki, A. et al. (2004) ARK5 is a tumor invasion-associated factor downstream of Akt signaling. Mol. Cell. Biol. 24, 3526-3535. Applications: Western Blotting
- Takahara, T. et al. (2006) J Biol Chem 281, 28605-14. Applications: Western Blotting
- Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91. Applications: Western Blotting
- Shavlakadze, T. et al. (2010) J Cell Sci Applications: Western Blotting
- Chen, C.H. et al. (2011) Sci Signal 4, ra10. Applications: Western Blotting
- Lee, J.W. et al. (2010) PLoS One 5, e15394. Applications: Western Blotting
- Fei, Z. et al. (2011) J Biol Chem 286, 27761-8. Applications: Western Blotting
- Yamada, O. et al. (2008) J Leukoc Biol 83, 1240-8. Applications: Western Blotting
PhosphoSitePlus®：
