Cell Cycle / Checkpoint Control
NEK7 (C34C3) Rabbit mAb
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #3057S | 100 μL | 46,000 | |
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NEK7抗体製品一覧
3057 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3057:
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Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのNEK7 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのNEK7 タンパク質のC末端領域（合成ペプチド） |
Western Blotting
Western blot analysis of GST-NEK6 and GST-NEK7 fusion proteins and extracts from HeLa and Jurkat cells using NEK7 (C34C3) Rabbit mAb.
The NEK family of protein kinases is composed of 11 members in humans that share an amino-terminal catalytic domain related to NIMA, a serine/threonine kinase identified in Aspergillus nidulans. While NIMA is critical for cell cycle progression in fungus, the function of NEK kinases in mammalian cells is largely unknown. NEK1 was first identified by screening mouse cDNA expression libraries and was demonstrated to have dual specificity kinase activity on both tyrosine and serine/threonine sites (1). NEK2 most closely resembles fungal NIMA in its primary structure and is believed to promote the splitting of duplicated centrosomes at the onset of mitosis (2,3). NEK3 is predominantly a cytoplasmic enzyme and its activity shows marginal variation throughout the cell cycle (4). NEK4 is ubiquitously expressed and its expression and subcellular location are not associated with cell cycle (5). NEK6/7 have been suggested to phosphorylate and activate p70 S6 kinase in vitro (6). Expression of an inactive NEK6 mutant arrests cells in M phase and interferes with chromosome segregation (7). NEK8 activity is not cell cycle regulated and may play a role in cell cycle independent microtubule dynamics (8). NEK9 is activated during mitosis and may participate in the activation of NEK6/7 during mitosis (9,10).
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Letwin, K. et al. (1992) EMBO J. 11, 3521-3531.
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Fry, A.M. et al. (1995) J. Biol. Chem. 270, 12899-12905.
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Fry, A.M. (2002) Oncogene 21, 6184-6194.
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Tanaka, K. and Nigg, E.A. (1999) J. Biol. Chem. 274, 13491-13497.
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Hayashi, K. et al. (1999) Biochem. Biophys. Res. Commun. 264, 449-456.
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Belham, C. et al. (2001) Curr. Biol. 11, 1155-1167.
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Yin, M.J. et al. (2003) J. Biol. Chem. 278, 52454-52460.
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Holland, P.M. et al. (2002) J. Biol. Chem. 277, 16229-16240.
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Belham, C. et al. (2003) J. Biol. Chem. 278, 34897-34909.
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Roig, J. et al. (2002) Genes Dev. 16, 1640-1658.
PhosphoSitePlus®：
NEK7