Chromatin Regulation / Acetylation
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #3147S | 100 μL | 46,000 | |
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EZH2抗体製品一覧
3147 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3147:
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Immunofluorescence
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、免疫蛍光細胞染色 (IF-IC) (1:100) |
| 特異性・感度 | |
| 内在性レベルのEzh2 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのEzh2 タンパク質の351-453番目の残基を含む組換えタンパク質 |
Western Blotting
Western blot analysis of extracts from various cell lines using Ezh2 (AC22) Mouse mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Ezh2 (AC22) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).
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Seller, W.B. and Loda, M. (2002) Cancer Cell 2, 349-350.
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Visser, H.P. et al. (2001) Br. J. Haematol. 112, 950-958.
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Chen, H. et al. (1996) Genomics 38, 30-37.
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Tonini, T. et al. (2004) Oncogene 23, 4930-4937.
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Muller, J. et al. (2002) Cell 111, 197-208.
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Kleer, C.G. et al. (2003) Proc Natl. Acad. Sci. USA 100, 11606-11611.
PhosphoSitePlus®：
