Jak/Stat Pathway
IFN-γ (3F1E3) Mouse mAb
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #3159S | 100 μL | 46,000 | |
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IFN-gamma抗体製品一覧
3159 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3159:
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ELISA Peptide
Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング（1:1,000）、免疫沈降（1:50）、ELISA（1:100） |
| 特異性・感度 | |
| ヒトの組み換えIFN-γ タンパク質を検出します。 |
| 使用抗原 | |
| 大腸菌に発現させ、Ni-NTAにより精製したヒトのIFN-γタンパク質 |
Western Blotting
Western blot analysis of lysates containing recombinant human IFN-γ using IFN-γ (3F1E3) Mouse mAb.
ELISA-Peptide
ELISA analysis of plates coated with recombinant human IFN-γ using IFN-γ (3F1E3) Mouse mAb.
Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression and immunoregulation (1). There are three naturally occurring interferons: α, β and γ. IFN-α is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-α from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-α induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway (2). IFN-α signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (β and ε) (3). All IFN-α signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.
IFN-γ, also known as type II interferon, is produced mainly in activated T lymphocytes and natural killer cells (4) and has broad effects on various cells of the immune system. Many signaling proteins including IL-2, FGF, and EGF induce the synthesis of IFN-γ.
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Stiehm, E.R. et al. (1982) Ann Intern Med 96, 80-93.
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Pellegrini, S. et al. (1989) Mol Cell Biol 9, 4605-12.
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Pfeffer, L.M. and Colamonici, O.R. (1991) Pharmacol Ther 52, 149-57.
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Young, H.A. and Hardy, K.J. (1995) J Leukoc Biol 58, 373-81.
PhosphoSitePlus®：
IFNG