Tyrosine Kinases / Adaptors
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #3161S | 100 μL | 57,000 | |
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| #3161L | 300 μL | 137,000 | |
PDGFR-b抗体製品一覧
推奨プロトコール
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3161:
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Flow
Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング(1:1,000)、フローサイトメトリー（1:100） |
| 特異性・感度 | |
| Tyr751 がリン酸化されたPDGF Receptor βを検出します。PDGF Receptor αを過剰発現させたときに交差反応する可能性があります。 |
| 使用抗原 | |
| ヒトのPDGF Receptor βのTyr751 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from various cell lines, untreated or PDGF-stimulated (40 ng/ml for 5 minutes), using Phospho-PDGF Receptor β (Tyr751) Antibody.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF-treated (green), using Phospho-PDGF Receptor β (Tyr751) Antibody compared to a nonspecific negative control antibody (red).
The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGF Receptor α homodimers bind all PDGF isoforms except those containing PDGF D. PDGF Receptor β homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGFα/β receptor binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as Grb2, Src, GAP, PI3 kinase, PLCγ and Nck. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ mediated PI3 kinase activation (8).
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