Angiogenesis
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #3175S | 100 μL | 46,000 | |
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PDGFRb抗体製品一覧
3175 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3175:
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Immunoprecipitation
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50) |
| 特異性・感度 | |
| 内在性レベルのPDGF Receptor βタンパク質を検出します。PDGF Receptor αタンパク質とは交差しません。 |
| 使用抗原 | |
| 組換えヒトPDGF Receptor βタンパク質 |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of NIH-3T3 cell lysates, using PDGF Receptor beta (2B3) Mouse mAb.
The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as Grb2, Src, GAP, PI3 kinase, PLCγ, and Nck. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8).
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Deuel, T.F. et al. (1988) Biofactors 1, 213-217.
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Bergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516.
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Betsholtz, C. et al. (2001) Bioessays 23, 494-507.
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Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 39-45.
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Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1-38.
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Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272.
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Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272.
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Kashishian, A. et al. (1992) EMBO J. 11, 1373-1382.
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