Tyrosine Kinases / Adaptors
- Tyrosine Kinases / Adaptors
PhosphoSitePlus®：
EGFR
- 推奨プロトコール
EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb |
イイネ！(4) |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #3197S | 100 μL | 65,000 | ログインすると国内在庫状況がご確認いただけます。
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3197 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途（希釈倍率） | |
|---|---|
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:200）、免疫組織染色（パラフィン）（1:100）、免疫蛍光細胞染色（IF-IC）（1:400）、免疫蛍光染色（IF-P）（1:800）、フローサイトメトリー（1:100） |
| 種交差性 | |
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| ヒト |
| 特異性・感度 | |
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| 内在性レベルのEGFR L858R 変異タンパク質を検出します。最適な特異性を得るには慎重に条件設定する必要がある場合があります。 |
| 検出タンパク質の分子量 | |
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| 175 kDa |
| 使用抗原 | |
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| ヒトのEGFR タンパク質のL858R 変異配列（合成ペプチド） |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from various EGFR expressing cell lines, showing the specificity of detection of mutant EGFR (L858R) protein using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper), and total EGFR control antibody (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded H1975 (left, EGFR L858R positive) and H1650 xenograft (right, EGFR deletion positive) using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma of known mutational status using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower). EGFR L858R positive lung (left), EGFR deletion positive lung sections (right).
Flow Cytometry
Flow cytometric analysis of two non-small cell lung cancer cell lines using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb. H3255 cells (green) harbor the L858R EGFR mutation while the H1650 cells (blue) have the exon 19 mutation (delE746-A750).
IF-IC
Confocal immunofluorescent analysis of H3255 (left), HCC827 (middle) and A431 cells (right) using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). H3255 cells have the L858R EGFR mutation, HCC827 cells have an exon 19 EGFR deletion, and A431 cells overexpress wild type EGFR. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-P
Confocal immunofluorescent analysis of paraffin-embedded H3255, H1975, HCC827 and H1650 xenografts using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper, green) and EGFR (D38B1) XP® Rabbit mAb #4267 (lower, green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
| バックグラウンド |
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The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.
EGFR is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Somatic mutations in the tyrosine kinase domain of EGFR are present in a subset of lung adenocarcinomas (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation L858R which occurs in exon 21 and short in-frame deletions in exon 19 (4,5). A common lesion in exon 19 is the deletion of E746-A750, although other variants occur.
- Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
- Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
- Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
- Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
- Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.
- Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
- Pao, W. et al. (2004) Proc Natl Acad Sci U S A 101, 13306-11.
- Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
- Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
- Riely, G.J. et al. (2006) Clin Cancer Res 12, 839-44.
| 使用文献 |
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- Kawahara, A. et al. (2010) Clin Cancer Res Applications: IHC-P (paraffin) Western Blotting
- Simonetti, S. et al. (2010) J Transl Med 8, 135. Applications: IHC-P (paraffin)
- Brevet, M. et al. (2010) J Mol Diagn 12, 169-76. Applications: IF-P (paraffin)
- Yu, J. et al. (2009) Clin Cancer Res 15, 3023-8. Applications: IF-P (paraffin) IHC-P (paraffin) Western Blotting
本製品は試験研究用です。