Adhesion
α-E-Catenin (23B2) Rabbit mAb
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #3240S | 100 μL | 46,000 | |
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Catenin-alpha抗体製品一覧
3240 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3240:
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Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング(1:1,000)、免疫沈降（1:50） |
| 特異性・感度 | |
| 内在性レベルの総α-E-Catenin タンパク質を検出します。この抗体は神経性のα-N-Cateninタンパク質交差反応すると思われます。 |
| 使用抗原 | |
| ヒトのα-E-Catenin タンパク質のN末端近傍領域由来の配列（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from HUVEC, NIH/3T3, MCF-7, ZR-75 and A431 cells using α-E-Catenin (23B2) Rabbit mAb.
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1).Recent evidence suggests that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).
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Kobielak, A. and Fuchs, E. (2004) Nat. Rev. Mol. Cell Biol. 5, 614-625.
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Yamada, S. et al. (2005) Cell 123, 889-901.
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Drees, F. et al. (2005) Cell 123, 903-915.
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Hwang, S.G. et al. (2005) J. Biol. Chem. 280, 12758-12765.
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