Translational Control
eIF3j Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #3261S | 100 μL | 46,000 | |
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3261 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3261:
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Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:50） |
| 特異性・感度 | |
| 内在性レベルのeIF3j タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのeIF3j タンパク質由来の配列（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from various cell types using eIF3j Antibody.
Translation initiation requires a set of factors to facilitate the association of the 40S ribosomal subunit with mRNA. The eIF4F complex, consisting of eIF4E, eIF4A and eIF4G, binds to the 5' cap structure of mRNA. eIF4F and eIF4B unwind the secondary structure of mRNA at its 5' untranslated region. The 40S ribosomal subunit, along with some initiation factors including eIF3, then binds to the 5' mRNA cap and searches along the mRNA for the initiation codon. eIF3 is a large translation initiation complex with 10 to 13 different subunits. eIF3a, eIF3b, eIF3c, eIF3e, eIF3f and eIF3h are the core subunits critical for the function of this complex. eIF3 physically interacts with eIF4G, which may be responsible for the association of the 40S ribosomal subunit with mRNA (1). eIF3 also stabilizes the binding of Met-tRNAf.eIF2.GTP to the 40S ribosomal subunit and helps keep the integrity of the resulting complex upon addition of the 60S ribosomal subunit (2). Studies have shown that mTOR interacts with eIF3 directly (3,4). When cells are stimulated by hormones or mitogenic signals, mTOR binds to the eIF3 complex and phosphorylates S6K1 (3). This process results in the dissociation of S6K1 from eIF3 and S6K1 activation. The activated S6K1 then phosphorylates its downstream targets including ribosomal protein S6 and eIF4B resulting in stimulation of translation. Further findings demonstrated that activated mTOR signaling induces the association of eIF3 with eIF4G upon stimulation with insulin (3).
One of the smallest subunits of eIF3, eIF3j, is critical in 40S initiation complex assembly (5). eIF3j has been shown to associate with the aminoacyl site and mRNA entry channel of the 40S ribosomal subunit (6). eIF3j has also been shown to have an additional role in the recycling of post-termination complexes (7).
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Masutani, M. et al. (2007) EMBO J 26, 3373-83.
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Chaudhuri, J. et al. (1999) J Biol Chem 274, 17975-80.
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Holz, M.K. et al. (2005) Cell 123, 569-80.
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Harris, T.E. et al. (2006) EMBO J 25, 1659-68.
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Fraser, C.S. et al. (2004) J Biol Chem 279, 8946-56.
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Fraser, C.S. et al. (2007) Mol Cell 26, 811-9.
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Pisarev, A.V. et al. (2007) Cell 131, 286-99.
PhosphoSitePlus®：
eIF3-alpha