Ca, cAMP & Lipid Signaling
Annexin A1 Antibody
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イイネ!(0)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #3299S | 100 μL | 46,000 | |
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Annexin A1抗体製品一覧
3299 の推奨プロトコール
最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注:各製品に最適化されたプロトコールをリンクしています。
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3299:
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Western Blotting
| 用途(希釈倍率) | |
| ウェスタンブロッティング(1:1,000) |
| 特異性・感度 | |
| 内在性レベルのヒトのAnnexin A1 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのAnnexin A1 タンパク質由来の配列(合成ペプチド) |
Western Blotting

Western blot analysis of extracts from various cell lines using Annexin A1 Antibody.
The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology (1). Annexin-1 (ANXA1) is the first characterized member of the annexin family of proteins and is able to bind to cellular membranes in a calcium-dependent manner, promoting membrane fusion and endocytosis (2-4). Annexin A1 has anti-inflammatory properties and inhibits phospholipase A2 activity (5,6). Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis and may be required for a late stage in inward vesiculation (7). Phosphorylation by PKC, EGFR, and Chak1 results in inhibition of annexin A1 function (8-10). Annexin A1 has also been identified as one of the 'eat-me' signals on apoptotic cells that are to be recognized and ingested by phagocytes (11). Annexin A1, as an endogenous anti-inflammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, apoptosis, and tumorigenesis and cancer development (12-14).
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Raynal, P. and Pollard, H.B. (1994) Biochim Biophys Acta 1197, 63-93.
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Blackwell, G.J. et al. (1980) Nature 287, 147-9.
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Rothhut, B. et al. (1983) Biochem Biophys Res Commun 117, 878-84.
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Hirata, F. et al. (1981) Proc Natl Acad Sci USA 78, 3190-4.
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Kim, K.M. et al. (1994) FEBS Lett 343, 251-5.
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Kim, S.W. et al. (2001) J Biol Chem 276, 15712-9.
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White, I.J. et al. (2006) EMBO J 25, 1-12.
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Varticovski, L. et al. (1988) Biochemistry 27, 3682-90.
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Dorovkov, M.V. and Ryazanov, A.G. (2004) J Biol Chem 279, 50643-6.
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Wang, W. and Creutz, C.E. (1994) Biochemistry 33, 275-82.
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Arur, S. et al. (2003) Dev Cell 4, 587-98.
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Perretti, M. and Gavins, F.N. (2003) News Physiol Sci 18, 60-4.
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Parente, L. and Solito, E. (2004) Inflamm Res 53, 125-32.
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Lim, L.H. and Pervaiz, S. (2007) FASEB J 21, 968-75.