Translational Control
GCN2 Antibody
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イイネ！(2)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #3302S | 100 μL | 46,000 | |
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GCN2抗体製品一覧
3302 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3302:
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Immunoprecipitation
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50) |
| 特異性・感度 | |
| リン酸化に関わらず内在性レベルのGCN2 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのGCN2 タンパク質のN末端近傍配列 (合成ペプチド) |
Western Blotting
Western blot analysis of extracts from ME180 and HT1376 cells that were untreated, treated with UV light (50mJ/cm2, 30 minutes), or subjected to nocodazole block (50 ng/ml, 24hrs), using GCN2 Antibody.
Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is a well documented mechanism of downregulating protein synthesis under a variety of stress conditions. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (1,2). GCN2 is also required for UV-light induced translation inhibition, and in vivo phosphorylation of murine GCN2 at Thr898 is induced by both UV irradiation and by leucine deprivation (3). UV-induced activation of NF-kappaB also requires GCN2, which may act simply by preventing translation of IkappaB-alpha to replace pools that have been ubiquitinated and degraded (4). Interestingly, proteasome inhibitors (MG132 and ALLN) activate the GCN2/eIF2alpha pathway, suggesting a pivotal role for this kinase in stress response and ubiquitin-mediated signaling (5). In vitro autophosphorylation of yeast GCN2 within its activation loop at Thr882 and Thr887 (Thr898 and Thr903 in mouse) has also been reported (6).
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Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
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Sheikh, M.S. and Fornace, A.J. (1999) Oncogene 18, 6121-6128.
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Deng, J. et al. (2002) Curr. Biol. 12, 1279-1286.
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Jiang, H.Y. and Wek, R.C. (2005) Biochem. J. 385, 371-380.
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Jiang, H.Y. and Wek, R.C. (2005) J. Biol. Chem. XXX, XXX-XXX.
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Garcia-Barrio, M. et al. (2002) J. Biol. Chem. 277, 30675-30683.
PhosphoSitePlus®：
GCN2