Chromatin Regulation / Acetylation
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #3314S | 100 μL | 46,000 | |
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JMJD1B抗体製品一覧
3314 の推奨プロトコール
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注：各製品に最適化されたプロトコールをリンクしています。
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3314:
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Immunofluorescence
Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:25）、免疫蛍光細胞染色（IF-IC）（1:200） |
| 特異性・感度 | |
| 内在性レベルのJMJD1B タンパク質の3つ全てのアイソフォームを検出します。HR、JMJD1A、JMJD1C などの他のJumonji C タンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのJMJD1B タンパク質由来の配列（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from HeLa and NCCIT cell lines using JMJD1B (C69G2) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using JMJD1B (C69G2) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen and thyroid receptors and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8).
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Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
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Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
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Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
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Cachon-Gonzalez, M.B. et al. (1994) Proc Natl Acad Sci USA 91, 7717-21.
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Ahmad, W. et al. (1998) Science 279, 720-4.
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Potter, G.B. et al. (2001) Genes Dev 15, 2687-701.
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Höög, C. et al. (1991) Mol Reprod Dev 30, 173-81.
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Yamane, K. et al. (2006) Cell 125, 483-95.
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Okada, Y. et al. (2007) Nature 450, 119-23.
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Loh, Y.H. et al. (2007) Genes Dev 21, 2545-57.
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Ko, S.Y. et al. (2006) Cell Struct Funct 31, 53-62.
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Hu, Z. et al. (2001) Oncogene 20, 6946-54.
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Lee, J.W. et al. (1995) Mol Endocrinol 9, 243-54.
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Wolf, S.S. et al. (2007) Arch Biochem Biophys 460, 56-66.
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Castermans, D. et al. (2007) Eur J Hum Genet 15, 422-31.
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