Neuroscience
TrkC (C44H5) Rabbit mAb
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #3376S | 100 μL | 46,000 | |
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3376 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3376:
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Immunofluorescence
Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:50）、免疫蛍光細胞染色（IF-IC）（1:1,600） |
| 特異性・感度 | |
| 内在性レベルのTrkC タンパク質を検出します。TrkA、TrkB とは交差しません。 |
| 検出タンパク質の分子量 | |
| 110 kDa、145 kDa |
| 使用抗原 | |
| ヒトのTrkC タンパク質のGly50 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from mouse and rat brain using TrkC (C44H5) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells, wild-type (upper left), NIH/3T3/TrkA (upper right), NIH/3T3/TrkB (lower left) or NIH/3T3/TrkC (lower right) using TrkC (C44H5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).
The phosphorylation sites are conserved between TrkA and TrkC: Tyr490 of TrkA corresponds to Tyr516 in TrkC, and Tyr674/675 of TrkA to Tyr709/710 in TrkC of the human sequence (7). Altered TrkC expression and corresponding gene mutations are seen in various forms of cancer, with increased expression a positive prognostic indicator in patients with medulloblastoma (8).
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Pierotti, M.A. and Greco, A. (2006) Cancer Lett 232, 90-8.
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Segal, R.A. and Greenberg, M.E. (1996) Annu Rev Neurosci 19, 463-89.
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Stephens, R.M. et al. (1994) Neuron 12, 691-705.
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Obermeier, A. et al. (1993) EMBO J 12, 933-41.
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Obermeier, A. et al. (1994) EMBO J 13, 1585-90.
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Yao, R. and Cooper, G.M. (1995) Science 267, 2003-6.
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Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42.
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Segal, R.A. et al. (1994) Proc Natl Acad Sci U S A 91, 12867-71.
PhosphoSitePlus®：
TrkC