Cell Cycle / Checkpoint Control
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
ご登録代理店情報 カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更は こちら。 |
| #3387S | 100 μL | 46,000 | |
|
3387 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
| | |
-
3387:
-
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのCdc6 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのCdc6 タンパク質のＮ末端近接領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from various cell types using Cdc6 (C42F7) Rabbit mAb.
The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome, and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to be bound to chromatin throughout the cell cycle (1,2). The pre-replication complex (Pre-RC) forms in late mitosis/early G1 phase beginning with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. The MCM complex is thought to be the replicative helicase, and formation of the pre-RC is referred to as chromatin licensing. Subsequent initiation of DNA replication requires the activation of the S-phase promoting kinases cdk2 and cdc7. Cdc7, which is active only in complex with its regulatory subunit dbf4, phosphorylates MCM proteins bound to chromatin and allows binding of the replication factor cdc45 and DNA polymerase (3,4).Replication licensing is controlled in part by the degradation of cdc6 in quiescent cells. Phosphorylation of cdc6 by cdk2 prevents its degradation, allowing pre-replication complexes to form (5). Cdc6 has recently been shown to play an important role in the intra-S-phase p21 Waf1/Cip1-dependent DNA damage response (6,7). Both cdc6 and CDT1 are degraded by the ubiquitin proteasome pathway in response to DNA damage associated with re-replication (8).
-
Okuno, Y. et al. (2001) EMBO J 20, 4263-77.
-
McNairn, A.J. et al. (2005) Exp Cell Res 308, 345-56.
-
Bell, S.P. and Dutta, A. (2002) Annu Rev Biochem 71, 333-74.
-
Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
-
Mailand, N. and Diffley, J.F. (2005) Cell 122, 915-26.
-
Kan, Q. et al. (2008) J Biol Chem 283, 17864-72.
-
Kan, Q. et al. (2008) Proc Natl Acad Sci USA 105, 4757-62.
-
Hall, J.R. et al. (2008) J Biol Chem 283, 25356-63.
PhosphoSitePlus®：
