Chromatin Regulation / Acetylation
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #3458S | 100 μL (50 tests) | 61,000 | |
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Histone H3抗体製品一覧
推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3458:
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Flow
Immunofluorescence
| 用途 (希釈倍率) | |
| 免疫蛍光細胞染色 (IF-IC) (1:400)、フローサイトメトリー (1:50) |
| 特異性・感度 | |
| 内在性レベルのSer10 がリン酸化されたHistone H3 タンパク質を検出します。他のリン酸化あるいはアセチル化されたHistone タンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのHistone H3 タンパク質のSer10 周辺領域 (合成リン酸化ペプチド) |
| Alexa Fluor®はMolecular Probes, Inc. の登録商標です。 |
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (A) or taxol-treated (B), stained with Phospho-Histone H3 (Ser10) (D2C8) XP™ Rabbit mAb (Alexa Fluor
®
647 Conjugate). The blue inserts represent PI (DNA) staining alone, showing an increase in the number of mitotic cells in the taxol-treated sample.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Phospho-Histone H3 (Ser10) (D2C8) XP™ Rabbit mAb (Alexa Fluor
®
647 Conjugate) (blue), β-Tubulin (9F3) Rabbit mAb (Alexa Fluor
®
555 Conjugate) #2116 (red) and Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP™ Rabbit mAb #2914 (green).
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).
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