PI3K / Akt Signaling
Phospho-NDRG1 (Ser330) Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #3506S | 100 μL | 57,000 | |
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NDRG1抗体製品一覧
3506 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3506:
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Immunoprecipitation
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100) |
| 特異性・感度 | |
| 内在性レベルのSer330 がリン酸化されたNDRG1 タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
| 46 kDa、48 kDa |
| 使用抗原 | |
| NDRG1 タンパク質のSer330 周辺領域 (合成リン酸化ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from Jurkat cells, treated with Calyculin A #9902 alone (100 nM for 20 min) or together with λ-phosphatase, using Phospho-NDRG1 (Ser330) Antibody.
NDRG1 (N-myc downstream-regulated gene 1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG1 family comprised of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates for SGK1 although the precise physiological role of this phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK3.
Phospho-NDRG1 (Ser330) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser330 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.
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Shimono, A. et al. (1999) Mech Dev 83, 39-52.
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Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
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van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
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Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
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Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
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Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
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Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
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Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
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Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
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Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
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Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
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Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
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Murray, J.T. et al. (2004) Biochem J 384, 477-88.
PhosphoSitePlus®：
NDRG1