PI3K / Akt Signaling
Phospho-GSK-3β (Thr390) Antibody
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イイネ！(1)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #3548S | 100 μL | 57,000 | |
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GSK3-beta抗体製品一覧
3548 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3548:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| 内在性レベルのThr390 がリン酸化されたGSK-3β タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのGSK-3β タンパク質のThr390 周辺領域 (合成リン酸化ペプチド) |
Western blot analysis of extracts from HeLa cells, either hydroxyurea-treated (4 mM, 20 hrs) to induce G1/S phase arrest or paclitaxel-treated (100 nM/ml, 20 hrs) for G2/M phase arrest, using Phospho-GSK-3β (Thr390) Antibody (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower) to show induction of phospho-GSK and equal loading.
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
Phosphorylation of Thr390 on GSK-3β was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery (6). The phosphorylation of this site was also reported by several other labs using phosphoproteomic analysis on mitotic cell extracts (7-10).
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Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279.
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Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141.
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Cross, D.A. et al. (1995) Nature 378, 785-789.
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Nusse, R. (1997) Cell 89, 321-323.
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Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.
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Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
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Daub, H. et al. (2008) Mol Cell 31, 438-48.
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Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
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Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
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Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci USA 101, 12130-5.
PhosphoSitePlus®：
GSK3B