Chromatin Regulation / Acetylation
DNMT3A (D23G1) Rabbit mAb
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イイネ！(1)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #3598S | 100 μL | 46,000 | |
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DNMT3a抗体製品一覧
3598 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3598:
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Immunoprecipitation
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100) |
| 特異性・感度 | |
| 内在性レベルのDNMT3A タンパク質を検出します。DNMT3B あるいは他のDNMT タンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのDNMT3A タンパク質のN末端領域 (合成ペプチド) |
Western Blotting
Western blot analysis of extracts from 293, NIH/3T3 and H-4-II-E cells using DNMT3A (D23G1) Rabbit mAb.
Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2 and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptional repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of DNA methylation and facilitate gene silencing (3-8). Improper DNA methylation contributes to diseased states such as cancer (1,2). Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic DNA correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas (9-12).
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Hermann, A. et al. (2004) Cell. Mol. Life Sci. 61, 2571-2587.
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Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell. Mol. Biol. Lett. 10, 631-647.
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Kim, G.D. et al. (2002) EMBO J. 21, 4183-4195.
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Fuks, F. et al. (2001) EMBO J. 20, 2536-2544.
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Geiman, T.M. et al. (2004) Biochem. Biophys. Res. Commun. 318, 544-555.
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Rountree, M.R. et al. (2000) Nat. Genet. 25, 269-277.
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Pradhan, S. and Kim, G.D. (2002) EMBO J. 21, 779-788.
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Fuks, F. et al. (2003) Nucleic Acids Res. 31, 2305-2312.
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Mizuno, S. et al. (2001) Blood 97, 1172-1179.
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Robertson, K.D. et al. (1999) Nucleic Acids Res. 27, 2291-2298.
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Xie, S. et al. (1999) Gene 236, 87-95.
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Kanai, Y. et al. (2001) Int. J. Cancer 91, 205-212.
PhosphoSitePlus®：
DNMT3A