Cell Cycle / Checkpoint Control
- Cell Cycle / Checkpoint Control
PhosphoSitePlus®：
MCM2
- 推奨プロトコール
MCM2 (D7G11) XP® Rabbit mAb |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #3619S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| #3619P | 40 μL for Custom Sampler Kit |  Custom Antibody Sampler Kitの構成品を選択できます。 5本以上を選択し、ページ右上のCartから製品確定書を発行してください。 尚、構成品の単品販売は致しておりません。 |
シグナル伝達研究応援キャンペーン プレゼント *Pサイズのみ
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3619 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 3619:
- ChIP Agarose ChIP Magnetic IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途（希釈倍率） | |
|---|---|
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:50）、免疫組織染色（パラフィン）（1:400）、免疫蛍光細胞染色（IF-IC）（1:400）、ChIP（1:25） |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル |
| 特異性・感度 | |
|---|---|
| 内在性レベルのMCM2 タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
|---|---|
| 125 kDa |
| 使用抗原 | |
|---|---|
| ヒトのMCM2 タンパク質のN末端周辺領域（合成ペプチド） |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from various cell types using MCM2 (D7G11) XP® Rabbit mAb.
IP
Immunoprecipitation of MCM2 from HeLa cell lysates using MCM2 (D7G11) XP® Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MCM2 (D7G11) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MCM2 (D7G11) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human small intestine using MCM2 (D7G11) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using MCM2 (D7G11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes, and either 20 μl of MCM2 (D7G11) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human TAP1 Promoter Primers #5148, human IRF-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
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The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and Cdt1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).
- Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
- Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
- Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
- Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
- Labib, K. et al. (2000) Science 288, 1643-7.
- Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
- Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
- Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
- Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.
| 使用例 | |
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本製品は試験研究用です。