TGF-beta/Smad Signaling
TGF-β Antibody
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イイネ！(2)
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #3711S | 100 μL | 46,000 | |
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TGF-beta抗体製品一覧
3711 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3711:
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Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:50） |
| 特異性・感度 | |
| 内在性レベルのTGF-β1、TGF-β2 および TGF-β3 タンパク質を検出します。また、TGF-β タンパク質の前駆体も検出します。 |
| 検出タンパク質の分子量 | |
| 12 kDa、25 kDa、45–65 kDa |
| 使用抗原 | |
| ヒトのTGF-β1 タンパク質のC末端配列（合成ペプチド） |
Western Blotting
Western blot analysis of recombinant human TGF-beta1, TGF-beta2 and TGF-beta3, using TGF-beta Antibody.
Western Blotting
Western blot analysis of extracts from K562, 293 and Raji cells, using TGF-beta Antibody.
Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).
There are three isoforms of TGF-beta designated TGF-beta1, TGF-beta2, and TGF-beta3, which are encoded by distinct genes and are expressed in a tissue specific manner (10). Each of the isoforms are synthesized as larger precursor proteins containing a propeptide region which gets cleaved prior to secretion from the cell. Mature TGF-beta contains two polypeptides linked linked by disulfide bonds forming a protein of about 25 kDa.
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Massague, J. et al. (2000) Cell 103, 295-309.
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Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
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Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
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Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
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Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
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López-Casillas, F. et al. (1991) Cell 67, 785-795.
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Wang, X.F. et al. (1991) Cell 67, 797-805.
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Derynck, R. et al. (1998) Cell 95, 737-740.
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Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178.
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Kingsley, D. M. et al. (1994) Genes Dev. 8, 133-146.
PhosphoSitePlus®：
TGFB1