Total抗体とリン酸化抗体のCSTジャパン株式会社

Tyrosine Kinases / Adaptors

CSTコード 包装
希望納入価格(円)
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2010年9月8日13時0分 現在
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EGFR抗体製品一覧

推奨プロトコール i

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推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

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用途 (希釈倍率)
ウェスタンブロッティング (1:1,000)、免疫組織染色 (パラフィン) (1:800)、免疫蛍光細胞染色 (IF-IC) (1:1,600)、フローサイトメトリー (1:800)
種交差性
ヒト、マウス、ラット、サル
特異性・感度
内在性レベルのTyr1068 がリン酸化されたEGF Receptor タンパク質を検出します。他のチロシンがリン酸化されたタンパク質とわずかに交差反応する可能性があります。
検出タンパク質の分子量
175 kDa
使用抗原
ヒトのEGF Receptor タンパク質のTyr1068 周辺領域 (合成リン酸化ペプチド)
抗体の由来
ウサギ
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts of BxPC-3 cells, untreated or EGF-stimulated, using Phospho-EGF Receptor (Tyr1068) (D7A5) XP™ Rabbit mAb (upper) and EGF Receptor Antibody #2232 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of SignalSlide ® Phospho-EGF Receptor IHC Controls #8102 [MDA-MB-468 cells control (left) or EGF-treated (right)] using Phospho-EGF Receptor (Tyr1068) (D7A5) XP™ Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HCC827 xenograft, control (left) or λ phosphatase-treated (right), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP™ Rabbit mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of A549 cells, untreated (blue) or EGF-treated (green), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP™ Rabbit mAb compared to concentration matched XP™ Rabbit (DA1E) mAb IgG Isotype Control #3900 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or EGF-treated (right), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP™ Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

バックグラウンド

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

  1. Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.
  2. Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.
  3. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.
  4. Hubbard, S.R. et al. (1994) Nature 372, 746-54.
  5. Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43.
  6. Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
  7. Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.
  8. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66.
  9. Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.
  10. Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.
使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
1110   Phospho-EGF Receptor (Tyr1068) Blocking Peptide
2231   Phospho-EGF Receptor (Tyr845) Antibody
2232   EGF Receptor Antibody
2234   Phospho-EGF Receptor (Tyr1068) Antibody
2235   Phospho-EGF Receptor (Tyr992) Antibody
2236   Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb
2237   Phospho-EGF Receptor (Tyr1045) Antibody
2238   Phospho-EGF Receptor (Ser1046/1047) Antibody
2239   EGF Receptor (1F4) Mouse mAb
2256   EGF Receptor (EGFR1) Mouse mAb (IP Specific)
2641   Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb
2646   EGF Receptor (C74B9) Rabbit mAb
2963   EGF Receptor (T43) Antibody
3056   Phospho-EGF Receptor (Thr669) Antibody
3265   EGF Receptor (V279) Antibody
4267   EGF Receptor (D38B1) XP™ Rabbit mAb
4404   Phospho-EGF Receptor (Tyr1148) Antibody
4405   EGF Receptor (15F8) Rabbit mAb
4407   Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb
7003   20X LumiGLO Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System (with Anti-rabbit IgG, HRP-linked Antibody)
7074   Anti-rabbit IgG, HRP-linked Antibody
7720   Prestained Protein Marker, Broad Range (Premixed Format)
7727   Biotinylated Protein Ladder Detection Pack
8102   SignalSlide Phospho-EGF Receptor IHC Controls
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9908   Epidermal Growth Factor (Human EGF)
9922   Phospho-EGF Receptor Antibody Sampler Kit

本製品は試験研究用です。

Phospho-EGF Receptor (Tyr1068) (D7A5) XP™ Rabbit mAb

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