Jak/Stat Pathway
SOCS1 (A156) Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #3950S | 100 μL | 46,000 | |
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SOCS1抗体製品一覧
3950 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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3950:
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Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのSOCS1 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのSOCS1 タンパク質のAla156 周辺領域（合成ペプチド） |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from BaF3 cells starved of mouse IL-3 and serum overnight, untreated (-) or mouse IL-3-treated (10 ng/ml, 6 hr; +) and NK-92 cells starved of human IL-2 and serum overnight, untreated (-) or human IL-2-treated (10 ng/ml, 6 hr; +), using SOCS1 (A156) Antibody.
Western Blotting
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with a construct overexpressing human SOCS1 (+), using SOCS1 (A156) Antibody.
The SOCS (suppressor of cytokine signaling) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified protein CIS1 (cytokine-inducible SH2-containing protein) as well as SOCS1 through SOCS7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation and immune responses.
SOCS1 (suppressor of cytokine signaling 1), also known as JAB (Janus Kinase binding protein), SSI-1 (Stat-induced Stat inhibitor-1), and TIP3 (Tec-interacting protein 3) (4-7), is a cytokine-regulated SOCS family member that directly inhibits Jak family members through interaction within their kinase activation loop. In addition to inhibiting Jak/Stat signaling, SOCS1 can also negatively regulate Toll-like receptors that contribute to innate immunity (8,9). The SOCS box of SOCS1 can trigger ubiquitin-mediated degradation of proteins within and outside of the Jak/Stat pathway (10,11). The highest expression of SOCS1 is seen in the thymus and spleen and it plays a critical role in T-cell activation and lymphocyte differentiation (4,12). SOCS1 also functions as a tumor suppressor protein by inhibiting hematopoietic oncogenes (13,14).
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Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
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Chen, X.P. et al. (2000) Immunity 13, 287-90.
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Hilton, D.J. et al. (1998) Proc Natl Acad Sci USA 95, 114-9.
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Starr, R. et al. (1997) Nature 387, 917-21.
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Endo, T.A. et al. (1997) Nature 387, 921-4.
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Naka, T. et al. (1997) Nature 387, 924-9.
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Ohya, K. et al. (1997) J Biol Chem 272, 27178-82.
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Kinjyo, I. et al. (2002) Immunity 17, 583-91.
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Davey, G.M. et al. (2006) Tissue Antigens 67, 1-9.
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Kamizono, S. et al. (2001) J Biol Chem 276, 12530-8.
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Rui, L. et al. (2002) J Biol Chem 277, 42394-8.
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Marine, J.C. et al. (1999) Cell 98, 609-16.
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De Sepulveda, P. et al. (1999) EMBO J 18, 904-15.
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Rottapel, R. et al. (2002) Oncogene 21, 4351-62.
PhosphoSitePlus®：
SOCS1