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Streptavidin-HRP |
イイネ！(3) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #3999S | 1 mL | 27,000 | ログインすると国内在庫状況がご確認いただけます。
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3999 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 3999:
- Western Blotting
| 用途 | |
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| ウェスタンブロッティング (1:2,000) |
| 種交差性 | |
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| すべての種 |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or treated with Paclitaxel #9807, using Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 and Streptavidin-HRP #3999 for detection.
| バックグラウンド |
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Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins (1,2).Chemiluminescent detection systems have emerged as the best all-around detection method for use with western blots and ELISA. These detection assays eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Streptavidin-HRP is used with biotinylated proteins and specific chemiluminescent substrates to generate light signal. Streptavidin-HRP conjugates have a very high turnover rate, coupling high sensitivity with short reaction times.
| 使用文献 |
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- Liang, J. et al. (2011) Mol Cell Biol 31, 2577-90. Applications: Western Blotting
| 関連製品 | |
|---|---|
| 7002 STOP Solution | |
| 7004 TMB Substrate | |
| 9808 Phosphate Buffered Saline (PBS-20X) | |
| 9809 Phosphate Buffered Saline with Tween 20 (PBST-20X) | |
| 9999 Nonfat Dry Milk |
本製品は試験研究用です。