Cell Cycle / Checkpoint Control
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年9月9日14時20分 現在 |
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| #4012S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途（希釈倍率） | |
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| ウェスタンブロッティング（1:1,000）、免疫沈降（1:50）、免疫組織染色（パラフィン）（1:50）、免疫蛍光細胞染色（IF-IC）（1:200） | |
| 種交差性 | |
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| ヒト、マウス、ラット、サル、ハムスター | |
| 特異性・感度 | |
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| 内在性レベルのMCM3 タンパク質を検出します。 | |
| 検出タンパク質の分子量 | |
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| 100 kDa | |
| 使用抗原 | |
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| ヒトのMCM3 タンパク質のN末端近接領域（合成ペプチド） | |
| 抗体の由来 | |
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| ウサギ | |
| 貯法 | |
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| -20℃ | |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from various cell types using MCM3 Antibody.
IP
Immunoprecipitation of MCM3 from HeLa cell lysates using MCM3 Antibody followed by western blot using the same antibody. Lane 1 is 5% input.
IHC-P (paraffin)
Immunohistochemcial analysis of paraffin-embedded human colon carcinoma using MCM3 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using MCM3 Antibody.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using MCM3 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
| バックグラウンド |
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The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2 through MCM7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and Cdt1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4 and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have also shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).
- Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
- Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
- Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
- Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
- Labib, K. et al. (2000) Science 288, 1643-7.
- Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
- Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
- Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
- Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。
