TGF-beta/Smad Signaling
NEDD4L Antibody
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イイネ！(1)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #4013S | 100 μL | 46,000 | |
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NEDD4L抗体製品一覧
4013 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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4013:
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Immunofluorescence
Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:50）、免疫蛍光細胞染色（IF-IC）（1:50） |
| 特異性・感度 | |
| 内在性レベルのNEDD4L タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
| 110 kDa, 135 kDa |
| 使用抗原 | |
| ヒトのNEDD4L タンパク質のGlu271 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from various cell lines using NEDD4L Antibody.
IF-IC
Confocal immunofluorescent analysis of C2C12 cells using NEDD4L Antibody (green). Blue pseudocolor = DRAQ5® #4084 (DNA fluorescent dye).
NEDD4 (neural precursor expressed, developmentally down-regulated protein 4) was originally identified as a gene that is highly expressed in the early mouse embryonic central nervous system (1). Subsequently, a family of NEDD4-like proteins have been defined that includes seven members in humans (2). NEDD4 and NEDD4-like (NEDD4L) proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4L have been shown to downregulate both neuronal voltage-gated Na channels (NaVs) and epithelial Na channels (ENaCs) in response to increased intracellular Na concentrations (3,4). The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins; ubiquitination of these proteins is mediated by the HECT domain of NEDD4 and results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's syndrome, an autosomal dominant form of hypertension (5). In addition to targeting sodium channels, NEDD4L has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation (6). Mouse and human NEDD4 are rapidly cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear (7).
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Kumar, S. et al. (1992) Biochem Biophys Res Commun 185, 1155-61.
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Harvey, K.F. and Kumar, S. (1999) Trends Cell Biol 9, 166-9.
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Dinudom, A. et al. (1998) Proc Natl Acad Sci USA 95, 7169-73.
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Goulet, C.C. et al. (1998) J Biol Chem 273, 30012-7.
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Staub, O. et al. (1996) EMBO J 15, 2371-80.
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Kuratomi, G. et al. (2005) Biochem J 386, 461-70.
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Harvey, K.F. et al. (1998) J Biol Chem 273, 13524-30.
PhosphoSitePlus®：
NEDD4L