Related Products
Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated)
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #4097S | 100 μL | 61,000 | |
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Mouse IgG抗体製品一覧
4097 の推奨プロトコール
最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注:各製品に最適化されたプロトコールをリンクしています。
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4097:
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Flow
Immunoprecipitation
IP

Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Akt (pan) (C67E7) Rabbit mAb #4691.
IP

Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated) #5102 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060.
Flow Cytometry

Flow cytometric analysis of Jurkat cells using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) (red).
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.