Neuroscience
- Neuroscience
PhosphoSitePlus®：
EGR1
- 推奨プロトコール
EGR1 (15F7) Rabbit mAb |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日11時35分 現在 |
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| #4153S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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4153 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 4153:
- ChIP Agarose ChIP Magnetic IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫組織染色 (パラフィン) (1:50)、免疫蛍光細胞染色 (IF-IC) (1:800)、ChIP (1:50) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、(ウシ) |
| 特異性・感度 | |
|---|---|
| 内在性レベルのEGR1 タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
|---|---|
| 75 kDa |
| 使用抗原 | |
|---|---|
| ヒトのEGR1 タンパク質のＮ末端由来の配列 (合成ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western analysis of extracts from serum starved PC12 cells, untreated or NGF-treated (100 ng/ml NGF for 2 hours), using EGR1 (15F7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brain using EGR1 (15F7) Rabbit mAb in the presence of control peptide (left) or EGR1 (15F7) Blocking Peptide #1015 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embeded human glioblastoma showing nuclear localization, using EGR1 (15F7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embeded human Non-Hodgkin's lymphoma, using EGR1 (15F7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brain, using EGR1 (15F7) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right) and labeled with EGR1 (44D5) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 THP-1 cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (30ng/ml) overnight and either 10 μl of EGR1 (15F7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ATAD2 promoter primers, SimpleChIP® Human EGR1 Promoter Primers #5549, human PCM1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
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EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3).
| 使用文献 |
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- Cui, H. et al. (2011) Biochem J Applications: Western Blotting
本製品は試験研究用です。