Translational Control
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #4202S | 100 μL | 46,000 | |
|
YB1抗体製品一覧
推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
| | |
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4202:
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IHC / Paraffin
Immunofluorescence
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫組織染色（パラフィン）（1:50）、免疫蛍光細胞染色（IF-IC）（1:100） |
| 種交差性 | |
| ヒト、マウス、ラット、サル、（ウシ、アフリカツメガエル） |
| 特異性・感度 | |
| 内在性レベルのYB1 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのYB1 タンパク質のC末端周辺領域（合成ペプチド） |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from various cell lines using YB1 (D299) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using YB1 (D299) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using YB1 (D299) Antibody (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5
®
#4084 (fluorescent DNA dye).
The Y-box binding protein 1 (YB1) belongs to a family of evolutionarily conserved, multifunctional Y-box proteins that bind single-stranded DNA and RNA and function as regulators of transcription, RNA metabolism, and protein synthesis (1). YB1 binds to Y-box sequences (TAACC) found in multiple gene promoters and can positively or negatively regulate transcription. YB1 activates genes associated with proliferation and cancer, such as cyclin A, cyclin B1, matrix metalloproteinase-2 (MMP-2), and the multi-drug resistance 1 (MDR1) gene (2-4). YB1 represses genes associated with cell death, including the Fas cell death-associated receptor and the p53 tumor suppressor gene (5-7). It also interacts with the RNA-splicing factor SRp30c and stabilizes interleukin 2 mRNA upon induction of T lymphocytes by interleukin 2 (8,9). The majority of YB1 protein localizes to the cytoplasm, with a minor pool found in the nucleus; however, nuclear localization appears to be critical for its role in promoting proliferation. Nuclear translocation is cell cycle-regulated, with YB1 protein accumulating in the nucleus during G1/S phase (2). In addition, nuclear translocation is induced in response to extracellular stimuli such as hyperthermia and UV irradiation, or treatment of cells with thrombin, interferons or insulin-like growth factor (IGF-1) (2,10). Treatment of the MCF-7 breast cancer cell line with IGF-1 results in Akt-mediated phosphorylation of YB1 on Ser102, which is required for nuclear translocation of YB1 and its ability to promote anchorage-independent growth (10). YB1 is over-expressed in many malignant tissues, including breast cancer, non-small cell lung carcinoma, ovarian adenocarcinomas, human osteosarcomas, colorectal carcinomas, and malignant melanomas. Nuclear YB1 expression correlates with high levels of proliferation, drug resistance, and poor tumor prognosis (2,7,10).
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