Neuroscience
Phospho-NMDAR2B (Tyr1070) Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #4209S | 100 μL | 57,000 | |
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NMDAR2B抗体製品一覧
4209 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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4209:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| 内在性レベルのTyr1070 がリン酸化されたNMDAR2B タンパク質を検出します。 |
| 使用抗原 | |
| NMDAR2B タンパク質のTyr1070 周辺領域 (合成リン酸化ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from rat brain, either sham-operated, 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-NMDAR2B (Tyr1070) Antibody (upper) or NMDAR2B #4207 Antibody (lower).
N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na and Ca2+ ions into the cell, and K out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).
Phosphorylation of NMDAR2B at Tyr1070 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of NMDAR2B at Tyr1070 was observed in extracts isolated from ischemic rat brain. For additional information please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org.
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Liu, X.B. et al. (2004) J. Neurosci. 24, 8885-8895.
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Westphal, R.S. et al. (1999) Science 285, 93-96.
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Tingley, W.G. et al. (1997) J. Biol. Chem. 272, 5157-5166.
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Hisatsune, C. et al. (1997) J. Biol. Chem. 272, 20805-20810.
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Raman, I.M. et al. (1996) Neuron 16, 415-421.
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Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.
PhosphoSitePlus®：
NMDAR2B