MAP Kinase Signaling
推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4370
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb |
イイネ！(5) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #4377S | 200 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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MAPK XP®モノクローナル抗体 | MAPK抗体製品一覧
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4377 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫蛍光細胞染色 (IF-IC) (1:200)、フローサイトメトリー (1:200) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、ミンク、ブタ、キイロショウジョウバエ、ゼブラフィッシュ |
| 特異性・感度 | |
|---|---|
| 内在性レベルのThr202/Tyr204 両方あるいはTyr204 だけがリン酸化されたp44 MAPK タンパク質 (Erk1) を検出します。また、内在性レベルのThr185/Tyr187 両方がリン酸化されたp42 MAPK タンパク質 (Erk2) も検出します。相応する残基がリン酸化されたSAPK/JNK あるいはp38 MAPK タンパク質とは交差しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 42 kDa、44 kDa |
| 使用抗原 | |
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| ヒトのp44 MAP Kinase のThr202/Tyr204 周辺領域 (合成リン酸化ペプチド) |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| 社内データ |
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推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4370
Western Blotting
Western blot analysis of purified MAPK phospho-proteins or extracts from NIH/3T3 cells treated with UV light and PDGF, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (upper), Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (middle), and Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 (lower).
Flow Cytometry
Flow cytometric analysis of U0126-inhibited (blue) or PMA-stimulated (green) Jurkat cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of NIH/3T3 cells untreated (left), inhibited with U0126 (MEK1/2 Inhibitor) #9903 (center), or stimulated with Platelet-Derived Growth Factor (PDGF) #9909 (right) and labeled with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
| バックグラウンド |
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Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
| 使用文献 |
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- Trampont, P. et al. (2006) Mol Cell Biol 26, 9035-44. Applications: Flow Cytometry
- Owaki, T. et al. (2006) J Immunol 177, 7579-87. Applications: Western Blotting
- Deane, J.A. et al. (2007) Blood 109, 2894-902. Applications: Flow Cytometry
- Mark, J.K. et al. (2008) J Biol Chem 283, 28574-83. Applications: Western Blotting
- Fehrenbacher, N. et al. (2008) Cancer Res 68, 6623-33. Applications: Western Blotting
- Wu, M. et al. (2009) Diabetes Metab Res Rev 25, 279-86. Applications: Western Blotting
- Chiron, D. et al. (2009) J Immunol 182, 4471-8. Applications: Western Blotting
- Gross, A.J. et al. (2009) J Immunol 182, 5382-92. Applications: Flow Cytometry
- Messal, N. et al. (2011) Eur J Immunol 41, 3443-54 Applications: Flow Cytometry
本製品は試験研究用です。