Tyrosine Kinases / Adaptors
- Tyrosine Kinases / Adaptors
PhosphoSitePlus®：
EGFR
- 推奨プロトコール
Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb |
イイネ！(1) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
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|---|---|---|---|---|
| #4407S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #4407L | 300 μL | 137,000 |
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4407 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫組織染色 (パラフィン) (1:250) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット |
| 特異性・感度 | |
|---|---|
| 内在性レベルのTyr1173 がリン酸化されたEGF Receptor を検出します。また、他の活性化した受容体型チロシンキナーゼと交差する可能性があります。 |
| 検出タンパク質の分子量 | |
|---|---|
| 175 kDa |
| 使用抗原 | |
|---|---|
| ヒトのEGF Receptor タンパク質のTyr1173 周辺領域 (合成リン酸化ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| 社内データ |
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Western Blotting
Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb specifically binds to tyrosine phosphorylated EGF receptors but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-EGF Receptor (Tyr1173)(53A5) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).
Western Blotting
Western blot analysis of extracts from BxPc-3 cells treated with EGF (100 ng/ml for 5 min) or EGF plus the EGF receptor tyrosine kinase inhibitor Iressa® (10 uM for 2 hrs), using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb (upper) or EGF Receptor Antibody #2232 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or calf-intestinal phosphatase (CIP) treated (right), using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb on SignalSlide™ Phospho-EGF Receptor IHC Controls #8102 (paraffin-embedded KYSE450 cell pellets, untreated (left) or EGF-treated (right)).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-EGF Receptor (Tyr1173)(53A5) Rabbit mAb (left) or the same antibody preincubated with Phospho-EGF Receptor (Tyr1173) Blocking Peptide (#1175) (right).
| バックグラウンド |
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The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
- Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.
- Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.
- Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.
- Hubbard, S.R. et al. (1994) Nature 372, 746-54.
- Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43.
- Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
- Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.
- Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66.
- Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.
- Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.
| 使用文献 |
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- Frogne, T. et al. (2009) Breast Cancer Res 11, R11. Applications: IHC-P (paraffin)
- Benedettini, E. et al. (2010) Am J Pathol 177, 415-23. Applications: Western Blotting
- Nishimura, M. et al. (2009) Mol Cell Biol 29, 5529-39. Applications: Western Blotting
本製品は試験研究用です。