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包装 |
希望納入価格 (円) |
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| #4408S | 250 μL | 24,000 | |
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Anti-Mouse IgG抗体製品一覧
| 用途（希釈倍率） | |
| 免疫蛍光染色（IF）（1:1,000）、フローサイトメトリー（1:1,000） |
| 情報 | |
| Anti-Mouse IgG (H+L), F(ab')2 Fragment を最適条件下でAlexa Fluor® 488 標識し、濃度を2 mg/mLに調製しました。ヒトのIgG と血清にて吸収してあります。 |
| Alexa Fluore®はMolecular Probes, Inc. の登録商標です。 |
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) (green) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SSEA1 (MC480) Mouse mAb #4744 detected with anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
High Content Screening
High content analysis of A439 cells exposed to varying concentrations of caffeine for 30 min prior to and 1.5 hr following a 100 mJ UV-treatment. With increasing concentrations of caffeine, a significant decrease (~2.5 fold) in phospho-p53 signal as compared to the UV-treated control was observed. When using phospho-p53 as a measurement, the IC50 of this compound was 2.95 mM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate).
Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
