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Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
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| #4409S | 250 μL | 24,000 | |
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Anti-Mouse IgG抗体製品一覧
| 用途（希釈倍率） | |
| 免疫蛍光染色（IF）（1:1,000）、フローサイトメトリー（1:1,000） |
| 情報 | |
| Anti-Mouse IgG (H+L), F(ab')2 Fragment を最適条件下でAlexa Fluor® 555 標識し、濃度を2 mg/mLに調製しました。ヒトのIgG と血清にて吸収してあります。 |
| Alexa Fluore®はMolecular Probes, Inc. の登録商標です。 |
IF-IC
Confocal immunofluorescent analysis of HeLa cells using S6 Ribosomal Protein (54D2) Mouse mAb #2317 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
High Content Screening
High content analysis of C2C12 cells exposed to varying concentrations of LY294002 #9901 for 2 hours. With increasing concentrations of LY294002, a significant decrease (~20 fold) in phospho-S6 Ribosomal protein (Ser235/236) signal as compared to the untreated control was observed. When using phospho-S6 Ribosomal protein as a measurement, the IC50 of this compound was 2.3 μM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate).
Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
