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| CSTコード |
包装 |
希望納入価格(円) |
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| #4410 | 250 μL | 24,000 | |
|
Anti-Mouse IgG抗体製品一覧
| 用途(希釈倍率) | |
| 免疫蛍光染色(IF)(1:1,000)、フローサイトメトリー(1:1,000) |
| 情報 | |
| Anti-Mouse IgG (H+L), F(ab')2 Fragment を最適条件下でAlexa Fluor® 647 標識し、濃度を2 mg/mLに調製しました。ヒトのIgG と血清にて吸収してあります。 |
| Alexa Fluore®はMolecular Probes, Inc. の登録商標です。 |
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-Mouse IgG (H+L), F(ab')
2
Fragment (Alexa Fluor
®
647 Conjugate) (green) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb #4545 detected with Anti-Mouse IgG (H+L), F(ab')
2
Fragment (Alexa Fluor
®
647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).
High Content Screening
High content analysis of C2C12 cells exposed to varying concentrations of U0126 #9903 for 2 hours. With increasing concentrations of U0126, a significant decrease (~20 fold) in phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) signal as compared to the untreated control was observed. When using Phospho-p44/42 MAPK (Erk1/2) protein as a measurement, the IC
50
of this compound was 2.5 μM. Data was generated on the Acumen
®
HCS platform using Anti-Mouse IgG (H+L), F(ab')
2
Fragment (Alexa Fluor® 647 Conjugate).
Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.