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Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) |
イイネ！(9) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #4412S | 250 μL | 24,000 | ログインすると国内在庫状況がご確認いただけます。
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| 由来 | |
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| ヤギ |
| 用途 (希釈倍率) | |
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| 免疫蛍光染色 (IF) (1:1,000)、フローサイトメトリー (1:1,000) |
| 情報 | |
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| Anti-Rabbit IgG (H+L), F(ab')2 Fragment を最適条件下でAlexa Fluor® 488 標識し、濃度を2 mg/mLに調製しました。 |
| 貯法 | |
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| 4℃ |
| Alexa Fluor®は、Molecular Probes, Inc. の登録商標です。 |
| 社内データ |
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Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951 and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) and compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of P19 cells using LIN28A (A177) Antibody #3978 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
High Content Screening
High content analysis of HeLa cells exposed to varying concentrations of staurosporine for 3hr. With increasing concentrations of staurosporine, a significant decrease (~2.5 fold) in phospho-MAPKAPK-2 signal as compared to the untreated control was observed. When using phospho-MAPKAPK-2 as a measurement, the IC50 of this compound was 92.5 mM. Data were generated on the Acumen HCS platform using Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate).
| バックグラウンド |
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Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。