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Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
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イイネ!(1)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #4413S | 250 μL | 24,000 | |
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Anti-Rabbit IgG抗体製品一覧
| 用途(希釈倍率) | |
| 免疫蛍光染色(IF)(1:1,000)、フローサイトメトリー(1:1,000) |
| 情報 | |
| Anti-Rabbit IgG (H+L), F(ab')2 Fragment を最適条件下でAlexa Fluor® 555 標識し、濃度を2 mg/mLに調製しました。ヒトとマウスの血清、プラズマサイトーマ/ハイブリドーマプロテイン、および精製ヒトパラプロテインで吸収してあります。 |
| Alexa Fluore®はMolecular Probes, Inc. の登録商標です。 |
IF-IC

Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb #9126 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-IC

Confocal immunofluorescent analysis of mouse cerebellum using α-Synuclein Antibody (IF Preferred) #2628 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) (red) and Neurofilament-L (DA2) Mouse mAb #2835 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
High Content Screening

High content analysis of A549 cells exposed to varying concentrations of LY294002 #9901 for 3 hrs, followed by 100 ng/mL EGF for 20 minutes. With increasing concentrations of LY294002, a significant decrease (~5 fold) in phospho-S6 Ribosomal Protein (Ser235/236) signal as compared to the uninhibited control was observed. When using phospho-S6 as a measurement, the IC50 of this compound was 3.06 μM. Data were generated on the Acumen HCS platform using Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate).
Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.