Cytoskeletal Signaling
| CSTコード | 包装 | 希望納入価格(円) |
||
|---|---|---|---|---|
| #4545 | 100μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
|
| 用途(希釈倍率) | |
|---|---|
| ウェスタンブロッティング(1:1,000)、免疫組織染色(パラフィン)(1:500)、免疫蛍光細胞染色(IF-IC)(1:400)、免疫蛍光染色(パラフィン)(1:50)、フローサイトメトリー(1:50) | |
| 種交差性 | |
|---|---|
| ヒト、ラット、サル | |
| 特異性・感度 | |
|---|---|
| 内在性レベルのKeratin 4、5、6、8、10、13 および 18 タンパク質を検出します。他のKeratin タンパク質とは交差しません。 | |
| 検出タンパク質の分子量 | |
|---|---|
| 46 kDa、55 kDa | |
| 使用抗原 | |
|---|---|
| A431細胞で発現させた Cytokeratin タンパク質 | |
| 抗体の由来 | |
|---|---|
| マウス | |
| 貯法 | |
|---|---|
| -20℃ | |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells, using Pan-Keratin (C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-P
Confocal immunofluorescent image of paraffin-embedded human colon carcinoma labeled with Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
| バックグラウンド |
|---|
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratins heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。

