Cytoskeletal Signaling
| CSTコード | 包装 | 希望納入価格(円) |
||
|---|---|---|---|---|
| #4548 | 100μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途(希釈倍率) | |
|---|---|
| ウェスタンブロッティング(1:2,000)、免疫組織染色(パラフィン)(1:500)、免疫細胞染色(IF-IC)(1:200)、フローサイトメトリー(1:1,000) | |
| 種交差性 | |
|---|---|
| ヒト | |
| 特異性・感度 | |
|---|---|
| 内在性レベルのKeratin 18 タンパク質を検出します。他のKeratin タンパク質とは交差しません。 | |
| 検出タンパク質の分子量 | |
|---|---|
| 46 kDa | |
| 使用抗原 | |
|---|---|
| ヒトのPMC-42 乳がん細胞 | |
| 抗体の由来 | |
|---|---|
| マウス | |
| 貯法 | |
|---|---|
| -20℃ | |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from HeLa and A431 cells, using Keratin 18 (DC10) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using Keratin 18 (DC10) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Keratin 18 (DC10) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human benign prostate hyperplasia, using Keratin 18 (DC10) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells, using Keratin 18 (DC10) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Immunofluorescent analysis of A431 cells, showing cytoplasmic staining, using Keratin 18 (DC10) Mouse mAb (left). The same cells were stained with Hoescht dye to mark nuclei (right).
| バックグラウンド |
|---|
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratins heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。

