MAP Kinase Signaling
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年9月8日13時0分 現在 |
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| #4631S | 200 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #4631L | 600 μL | 137,000 |
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推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫組織染色 (パラフィン) (1:50)、免疫蛍光細胞染色 (IF-IC) (1:100) | |
| 種交差性 | |
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| ヒト、マウス、ラット、サル、キイロショウジョウバエ、 (ミンク、ゼブラフィッシュ、ハムスター) | |
| 特異性・感度 | |
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| 内在性レベルのThr180/Tyr182 がリン酸化されたp38 MAPK を検出します。リン酸化p42/44 MAPK (Erk1/2) およびSAPK/JNK タンパク質とは交差しません。 | |
| 検出タンパク質の分子量 | |
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| 43 kDa | |
| 使用抗原 | |
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| ヒトのp38 MAPK のThr180/Tyr182 周辺領域 (合成リン酸化ペプチド) | |
| 抗体の由来 | |
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| ウサギ | |
| 貯法 | |
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| -20℃ | |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from Jurkat, NIH/3T3 and PC12 cells, untreated or treated as indicated, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemistry of paraffin-embedded lung carcinoma, showing nuclear localization, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemistry of paraffin-embedded colon carcinoma, showing nuclear localization, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemistry of paraffin-embedded human glioblastoma, untreated (left) or CIP phosphatase-treated (right), using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemistry of paraffin-embedded breast carcinoma, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb (left) or the same antibody preincubated with antigen phospho-peptide (right).
IHC-P (paraffin)
Immunohistochemistry of paraffin-embedded NIH/3T3 cells, untreated (left) or anisomycin-treated (right), using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells -/+ UV light, labeled with Phospho-p38 MAPK (green). Absence of staining in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).
Table
| バックグラウンド |
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p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Han, J. et al. (1994) Science 265, 808-811.
- Lee, J.C. et al. (1994) Nature 372, 739-746.
- Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
- Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
| 使用文献 |
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- Miyaji, M. et al. (2009) Proc Natl Acad Sci U S A 106, 14502-7. Applications: Western Blotting
本製品は試験研究用です。
