MAP Kinase Signaling
p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #4696S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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4696 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途（希釈倍率） | |
|---|---|
| ウェスタンブロッティング（1:2,000）、免疫組織染色（パラフィン）（1:500）、免疫蛍光細胞染色（IF-IC）（1:50）、フローサイトメトリー（1:200） |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、ミンク、ブタ、ゼブラフィッシュ |
| 特異性・感度 | |
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| 内在性レベルのp44/42 MAP Kinase を検出します。いくつかの手法ではp44/42 MAP Kinase より簡単にp42/Erk2 を認識します。JNK/SAPK および p38 MAP Kinase とは交差しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 42 kDa、44 kDa |
| 使用抗原 | |
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| ラットのp44/42 MAP Kinase の配列（合成ペプチド） |
| 抗体の由来 | |
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| マウス |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from NIH/3T3, PC12 and COS cells, using p44/42 MAP Kinase (L34F12) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using p44/42 MAP Kinase (L34F12) Mouse mAb in the presence of control peptide (left) or p44/42 MAP Kinase Blocking Peptide (#4696 Specific) #1245 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic and nuclear localization, using p44/42 MAP Kinase (L34F12) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using p44/42 MAP Kinase (L34F12) Mouse mAb compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells, either U0126-treated (#9903, 10 μM, 2 hour, left) or PDGF-treated (#9909, 100 ng/ml, 20 min, right), using p44/42 MAP Kinase (L34F12) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
| バックグラウンド |
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Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
| 使用文献 |
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- Morigi, M. et al. (2006) Am J Pathol 169, 1965-75. Applications: Western Blotting
- Kosako, H. et al. (2009) Nat Struct Mol Biol 16, 1026-35. Applications: Western Blotting, IF-IC
本製品は試験研究用です。