Cell Cycle / Checkpoint Control
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #4731S | 100 μL | 46,000 | |
|
ORC1抗体製品一覧
| 用途（希釈倍率） | |
| ウエスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのORC1 タンパク質を検出します。他のORC サブユニットとは交差しません。 |
| 検出タンパク質の分子量 | |
| 95 kDa（マウス）、100 kDa |
| 使用抗原 | |
| His6で標識された組換えヒトORC1 タンパク質 |
Western Blotting
Western blot analysis of extracts from various cell lines using ORC1 (7A7) Rat mAb.
The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7).
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Machida, Y.J. et al. (2005) J. Biol. Chem. 280, 27624-27630.
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Baltin, J. et al. (2006) J. Biol. Chem. 281, 12428-12435.
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Gibson, D.G. et al. (2006) Genes Cells 11, 557-573.
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Ramachandran, L. et al. (2006) FEMS Yeast Res. 6, 763-776.
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Rowles, A. and Blow, J.J. (1997) Curr. Opin. Genet. Dev. 7, 152-157.
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DePamphilis, M.L. (2003) Gene 310, 1-15.
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McNairn, A.J. et al. (2005) Exp. Cell. Res. 308, 345-356.
PhosphoSitePlus®：
