Apoptosis and Autophagy
Max (S20) Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #4739S | 100 μL | 46,000 | |
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Max抗体製品一覧
4739 の推奨プロトコール
最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注:各製品に最適化されたプロトコールをリンクしています。
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4739:
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Immunofluorescence
Western Blotting
| 用途(希釈倍率) | |
| ウェスタンブロッティング(1:1,000)、免疫蛍光細胞染色(IF-IC)(1:100) |
| 特異性・感度 | |
| 内在性レベルのMax タンパク質を検出します。 |
| 使用抗原 | |
| Max タンパク質のN末端近傍領域(合成ペプチド) |
Western Blotting

Western blot analysis of extracts from various cell lines using Max (S20) Antibody.
Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with human Max contruct, using Max (S20) Antibody.
IF-IC

Confocal immunofluorescent analysis of ACHN cells using Max (S20) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).
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Baudino, T.A. and Cleveland, J.L. (2001) Mol. Cell. Biol. 21, 691-702.
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Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-1217.
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Henriksson, M. and Lüscher, B. (1996) Adv. Cancer Res. 68, 109-182.
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Grandori, C. et al. (2000) Annu. Rev. Cell Dev. Biol. 16, 653-699.